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Echelon提供各种抗体、分析检测试剂盒和抑制剂等产品,尤其擅长以细胞信号、脂类代谢和标记物研究为主的相关产品。为研究治疗诸如癌症、糖尿病、炎症、感染和心脑血管疾病专家提供全面有效工具。
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echelon-inc/B-Catenin/Tcf Protein-Protein Interaction FP Assay/1kit/K-5900-1kit

echelon-inc/B-Catenin/Tcf Protein-Protein Interaction FP Assay/1kit/K-5900-1kit
  • echelon-inc/B-Catenin/Tcf Protein-Protein Interaction FP Assay/1kit/K-5900-1kit
商品介绍

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CatalogNo.: K-5900

Description: TheK-5900kithasbeendiscontinued.PleasecontactEcheloninindividualkitreagentsareneeded.



Description:Echelon’sβ-catenin/Tcfprotein-proteininteractionassayisafluorescencepolarization(FP)assaydesignedforhighthroughputscreeningina384-wellformatforsmallmoleculeinhibitionortheβ-catenin:Tcfprotein-proteininteraction.

β-cateninisakeymediatorfortheWntpathway.TheWntpathwayisclassifiedasaβ-catenin-dependentpathwayorβ-catenin-independentpathway.TheWnt/β-catenin-dependentpathwayisresponsIBLeformanycellularregulationssuchasproliferation,differentiationandcell-cellcommunication.HyperactivatedWnt/β-catenin-dependentpathwaytriggersprogressionofmanycancerssuchascolorectalcancers,livercancer,breastcancer,etc.ThetranscriptionoftheoveractiveWnt/β-catenin-dependentpathwayissolelydependentonthenuclearβ-catenin:T-cellfactor(Tcf)binding.Thus,inhibitorstargetingtheβ-catenin/Tcfprotein-proteininteractionappeartobeapromisingtherapeuticstrategyforcancer.Lately,literaturesuggestsactivationoftheβ-catenin/Tcfsignalingbythelipidgrowthfactor,lysophosphatidicacid(LPA),maybeinvolvedinneurogenesisaswell.

Echelon’sβ-catenin/Tcfprotein-proteininteractionassayisafluorescencepolarization(FP)assaydesignedforhighthroughputscreeningina384-wellformatforsmallmoleculeinhibitionortheβ-catenin:Tcfprotein-proteininteraction.Theβ-cateninproteinusedinthisassayisapartialproteinwhichincludesthedomainthatbindstothehumanTcf.AselectedshortpeptidesequenceofthehumanTcfwithTAMRAlabel(Tcf-TAMRA)isusedintheassay.Whenβ-cateninbindstotheTcf-TAMRA,theTAMRAfluorophore’srotationalmovementbecomesslowerduetotheincreaseinmolecularmassandthustheemittedlightremainspolarized(figure1).Therefore,theinhibitionbetweenβ-cateninandTcf-TAMRAbindingcanbemonitoredbythechangeinpolarizationoflight.PKF118-310(Lepourcelet2004)isincludedintheassayasacontrolinhibitor.

Storage

Uponreceipt,storekitat-20°C.Allowthereagentstowarmtoroomtemperature.Centrifugevialsbeforeopening.

References

1)Lepourcelet,M,Chen,YN,etal.SmallmoleculeantagoNISTsoftheoncogenicTcf/beta-cateninproteincomplex.CancerCell.5(1),91-102(2004).
2)Zhang,M,Huang,Z,etal.Newhomogeneoushigh-throughputassaysforinhibitorsof?-catenin/Tcfprotein–proteininteractions.AnalBiochem.424(1),57-63(2012).>

品牌介绍

Echelon Biosciences Incorporated (EBI) was incorporated under the laws of the state of Utah as Echelon Research Laboratories, Inc. on October 3, 1997. Initially, EBI was formed from a project under the Center for Cell Signaling, a State of Utah Center of Excellence, with the aim to further the science of lipid cell signaling and critical metabolic enzymes, which provide new and viable targets for the development of potential drugs and diagnostics for cancer, diabetes, inflammation, infections, and cardiovascular disease.

From 1997 through 2004, EBI primarily used government grants and product sales revenues to fund its research efforts. In early 2005, EBI was purchased by Aeterna Zentaris (NAS: AEZS) for its MEP anti-infectives, PI3-Kinase small molecule program, catalog business, and scientific expertise. In late 2007, Frontier Scientific Incorporated (FSI) based in Logan, Utah purchased EBI. Specializing in porphyrin and boron chemistry, FSI opened the doors for expanded research.

Since 1997, EBI has retained its commitment to developing novel assays and reagents for lipid research and beyond. At EBI’s core is the desire to innovate, thus pushing medicinal research to new limits. We look forward to providing scientists around the world with the tools they need to cure human disease states for years to come and we welcome any suggestions about how we can better do so.



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Echelon特色产品

  • PI3-Kinase PI3-
  • Lysolipids 血溶性
  • Sphingolipids 
  • Autotaxin(ATX) 自分泌运动因子

Echelon产品线

 

Lipid Kinases & Phosphatases

质激和磷

Lipid-Protein Interaction Tools

蛋白质相互作用工具

Lipid-Specific Assay Kits

质特定检测试剂

Lipid-Specific Antibodies & Binding Proteins

质特定抗体和结合蛋白

Hyaluronic Acid & CollaGEn IV Assay Kits

透明质IV型胶原检测试剂

Synthetic Inositol phosphates, Lipids, & Phospholipids

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